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2/25/2022 12:19:16 PM | Browse: 286 | Download: 514
Publication Name World Journal of Stem Cells
Manuscript ID 64840
Country/Territory Pakistan
Received
2021-02-28 09:26
Peer-Review Started
2021-02-28 09:33
To Make the First Decision
Return for Revision
2021-04-19 18:44
Revised
2021-07-29 10:40
Second Decision
2022-01-06 03:31
Accepted by Journal Editor-in-Chief
Accepted by Company Editor-in-Chief
2022-01-06 19:43
Articles in Press
2022-01-06 19:43
Publication Fee Transferred
Edit the Manuscript by Language Editor
2021-12-11 20:11
Typeset the Manuscript
2022-02-22 01:39
Publish the Manuscript Online
2022-02-25 12:19
ISSN 1948-0210 (online)
Open Access
Copyright ©The Author(s) 2022. Published by Baishideng Publishing Group Inc. All rights reserved.
Article Reprints For details, please visit: http://www.wjgnet.com/bpg/gerinfo/247
Permissions For details, please visit: http://www.wjgnet.com/bpg/gerinfo/207
Publisher Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA
Website http://www.wjgnet.com
Category Cell Biology
Manuscript Type Basic Study
Article Title Transcription regulators differentiate mesenchymal stem cells into chondroprogenitors and their in vivo implantation regenerated the intervertebral disc degeneration
Manuscript Source Invited Manuscript
All Author List Shumaila Khalid, Sobia Ekram, Asmat Salim, G Rasul Chaudhry and Irfan Khan
ORCID
Author(s) ORCID Number
Shumaila Khalid http://orcid.org/0000-0002-4523-6936
Sobia Ekram http://orcid.org/0000-0003-0073-8729
Asmat Salim http://orcid.org/0000-0001-5181-0458
G Rasul Chaudhry http://orcid.org/0000-0003-1692-8420
Irfan Khan http://orcid.org/0000-0003-1878-7836
Funding Agency and Grant Number
Funding Agency Grant Number
Higher Education Commission Pakistan 7083
Corresponding Author Irfan Khan, PhD, Assistant Professor, Dr. Panjwani Center for Molecular Medicine and Drug Research, International Center for Chemical and Biological Sciences, University of Karachi, KU Circular Rd, Karachi 75270, Sindh, Pakistan. khan@iccs.edu
Key Words Intervertebral Disc Degeneration; Human Umbilical Cord; Transcription Factors; Mesenchymal Stem Cells; Gene Expression; Regeneration
Core Tip In this study, we highlighted that overexpression of chondrogenic transcription factors in human umbilical cord derived mesenchymal stem cells (hUC-MSCs) accelerated their differentiation potential into chondroprogenitor cells. The synergistic effect of Sox-9, and Six-1 transcription factors leads the MSCs to differentiate into chondrogenic cells in the basal medium, which produced the same effect as the chondro-induction medium. The in vivo transplantation of these transfected cells leads to their homing, integration, and differentiation into nucleus pulposus cells of the intervertebral disc. Histological observation and grading score showed that the cellular transplanted group significantly regenerated the degenerated disc. This approach of manipulating stem cells in vitro and their transplantation in the degenerated disc could help to develop a better treatment option for treating degenerative disc diseases.
Publish Date 2022-02-25 12:19
Citation Khalid S, Ekram S, Salim A, Chaudhry GR, Khan I. Transcription regulators differentiate mesenchymal stem cells into chondroprogenitors and their in vivo implantation regenerated the intervertebral disc degeneration. World J Stem Cells 2022; 14(2): 163-182
URL https://www.wjgnet.com/1948-0210/full/v14/i2/163.htm
DOI https://dx.doi.org/10.4252/wjsc.v14.i2.163
Full Article (PDF) WJSC-14-163.pdf
Full Article (Word) WJSC-14-163.docx
Manuscript File 64840_Auto_Edited-JLW-Filipodia.docx
Answering Reviewers 64840-Answering reviewers.pdf
Audio Core Tip 64840-Audio core tip.m4a
Biostatistics Review Certificate 64840-Biostatistics statement.pdf
Conflict-of-Interest Disclosure Form 64840-Conflict-of-interest statement.pdf
Copyright License Agreement 64840-Copyright license agreement.pdf
Approved Grant Application Form(s) or Funding Agency Copy of any Approval Document(s) 64840-Grant application form(s).pdf
Institutional Animal Care and Use Committee Approval Form or Document 64840-Institutional animal care and use committee statement.pdf
Institutional Review Board Approval Form or Document 64840-Institutional review board statement.pdf
Non-Native Speakers of English Editing Certificate 64840-Language certificate.pdf
Peer-review Report 64840-Peer-review(s).pdf
Scientific Editor Work List 64840-Scientific editor work list.pdf