| ISSN |
2307-8960 (online) |
| Open Access |
This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/Licenses/by-nc/4.0/ |
| Copyright |
©The Author(s) 2022. Published by Baishideng Publishing Group Inc. All rights reserved. |
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| Publisher |
Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA |
| Website |
http://www.wjgnet.com |
| Category |
Gastroenterology & Hepatology |
| Manuscript Type |
Clinical and Translational Research |
| Article Title |
Single-cell RNA-sequencing combined with bulk RNA-sequencing analysis of peripheral blood reveals the characteristics and key immune cell genes of ulcerative colitis
|
| Manuscript Source |
Unsolicited Manuscript |
| All Author List |
Yan-Cheng Dai, Dan Qiao, Chen-Ye Fang, Qiu-Qin Chen, Ren-Ye Que, Tie-Gang Xiao, Lie Zheng, Li-Juan Wang and Ya-Li Zhang |
| ORCID |
|
| Funding Agency and Grant Number |
| Funding Agency |
Grant Number |
| the National Natural Science Foundation of China |
81873253 |
| the National Natural Science Foundation of China |
81704009 |
| the Shanghai Natural Science Foundation |
22ZR1458800 |
| the Hongkou District Health Committee |
HKZK2020A01 |
| the Xinglin Scholar Program of Shanghai University of Traditional Chinese Medicine, The document of the Shanghai University of Traditional Chinese Medicine |
2020 No.23. |
|
| Corresponding Author |
Ya-Li Zhang, PhD, Research Fellow, Institute of Digestive Diseases, LongHua Hospital Shanghai University of Traditional Chinese Medicine, No. 725 South Wanping Road, Shanghai 200032, China. zhanghappy181015@126.com |
| Key Words |
Ulcerative colitis; Single-cell RNA-seq; Bulk RNA-seq; Peripheral blood; Key genes |
| Core Tip |
Using the Gene Expression Omnibus database, we analyzed peripheral blood cell subtypes of patients with ulcerative colitis (UC) by single-cell RNA sequencing (scRNA-seq) combined with bulk RNA sequencing (RNA-seq) to reveal the core genes of UC. Through scRNA-seq analysis, least absolute shrinkage and selection operator diagnostic model building and weighted gene correlation network analysis, we identified RhoB, cathepsin D and zyxin as core genes of UC that are closely related to monocyte infiltration that may serve as diagnostic markers and molecular targets for UC therapeutic intervention. |
| Publish Date |
2022-11-23 09:44 |
| Citation |
Dai YC, Qiao D, Fang CY, Chen QQ, Que RY, Xiao TG, Zheng L, Wang LJ, Zhang YL. Single-cell RNA-sequencing combined with bulk RNA-sequencing analysis of peripheral blood reveals the characteristics and key immune cell genes of ulcerative colitis. World J Clin Cases 2022; 10(33): 12116-12135 |
| URL |
https://www.wjgnet.com/2307-8960/full/v10/i33/12116.htm |
| DOI |
https://dx.doi.org/10.12998/wjcc.v10.i33.12116 |
