ISSN |
1007-9327 (print) and 2219-2840 (online) |
Open Access |
This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
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Copyright |
© The Author(s) 2016. Published by Baishideng Publishing Group Inc. All rights reserved. |
Article Reprints |
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Permissions |
For details, please visit: http://www.wjgnet.com/bpg/gerinfo/207
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Publisher |
Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA |
Website |
http://www.wjgnet.com |
Category |
Oncology |
Manuscript Type |
Basic Study |
Article Title |
Tumor-specific expression of shVEGF and suicide gene as a novel strategy for esophageal cancer therapy
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Manuscript Source |
Unsolicited Manuscript |
All Author List |
Ting Liu, Hai-Jun Wu, Yu Liang, Xu-Jun Liang, Hui-Chao Huang, Yan-Zhong Zhao, Qing-Chuan Liao, Ya-Qi Chen, Ai-Min Leng, Wei-Jian Yuan, Gui-Ying Zhang, Jie Peng and Yong-Heng Chen |
Funding Agency and Grant Number |
Funding Agency |
Grant Number |
National Natural Science Foundation of China |
81372904 |
National Natural Science Foundation of China |
81272971 |
National Natural Science Foundation of China |
81272735 |
National Natural Science Foundation of China |
30800518 |
Science and Technology Department of Hunan Province |
2010CK3013 |
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Corresponding Author |
Yong-Heng Chen, MD, PhD, Key Laboratory of Cancer Proteomics of Chinese Ministry of Health, XiangYa Hospital, Central South University, No. 87 Xiangya Road, Changsha 410008, Hunan Province,
China. yonghenc@163.com
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Key Words |
Esophageal cancer; Suicide gene; RNA interference; Vascular endothelial growth factor; Nanoparticles |
Core Tip |
Esophageal cancer is a highly aggressive neoplasm with poor prognosis and low survival rates. In this study, an expression vector carrying a fusion suicide gene (yCDglyTK) and shRNA against vascular endothelial growth factor (VEGF) was constructed and delivered into EC9706 esophageal cancer cells by calcium phosphate nanoparticles (CPNP). To achieve tumor selectivity, the expression of the fusion suicide gene was driven by a tumor-specific human telomerase reverse transcriptase promoter. Our results showed that the novel expression vector was efficiently introduced into EC9706 cells by CPNP, leading to cellular expression of yCDglyTK and decreased VEGF level. With exposure to 5-fluorocytosine, it exhibited strong anti-tumor effects against esophageal cancer both in vitro and in vivo. Combination of VEGF shRNA with the fusion suicide gene demonstrated strong anti-cancer effects. Our study provides a novel approach for esophageal cancer-targeted gene therapy.
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Publish Date |
2016-06-13 09:14 |
Citation |
Liu T, Wu HJ, Liang Y, Liang XJ, Huang HC, Zhao YZ, Liao QC, Chen YQ, Leng AM, Yuan WJ, Zhang GY, Peng J, Chen YH. Tumor-specific expression of shVEGF and suicide gene as a novel strategy for esophageal cancer therapy. World J Gastroenterol 2016; 22(23): 5342-5352 |
URL |
http://www.wjgnet.com/1007-9327/full/v22/i23/5342.htm |
DOI |
http://dx.doi.org/10.3748/wjg.v22.i23.5342 |