| ISSN |
2222-0682 (online) |
| Open Access |
This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/ |
| Copyright |
© The Author(s) 2016. Published by Baishideng Publishing Group Inc. All rights reserved. |
| Article Reprints |
For details, please visit: http://www.wjgnet.com/bpg/gerinfo/247
|
| Permissions |
For details, please visit: http://www.wjgnet.com/bpg/gerinfo/207
|
| Publisher |
Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA |
| Website |
http://www.wjgnet.com |
| Category |
Methodology |
| Manuscript Type |
Basic Study |
| Article Title |
Rat model of cholelithiasis with human gallstones implanted in cholestasis-induced virtual gallbladder
|
| Manuscript Source |
Invited Manuscript |
| All Author List |
Marlein Miranda Cona, Yewei Liu, Ting Yin, Yuanbo Feng, Feng Chen, Stefaan Mulier, Yue Li, Jian Zhang, Raymond Oyen and Yicheng Ni |
| Funding Agency and Grant Number |
| Funding Agency |
Grant Number |
| Flanders Research Foundation |
FWO-42865 |
| KU Leuven Molecular Small Animal Imaging Center MoSAIC |
KUL EF/05/08 |
| Center of excellence in vivo molecular imaging research |
|
| KU Leuven project |
IOF-HB/08/009 |
| KU Leuven project |
IOF-HB/12/018 |
| National Natural Science Foundation of China |
81071828 |
| European Union (Asia-Link CfP 2006-EuropeAid/123738/C/ACT/Multi-Proposal) |
128-498/111 |
| Jiangsu Province Natural Science Foundation |
BK2010594 |
|
| Corresponding Author |
Dr. Yicheng Ni, MD, PhD, Professor, Department of Imaging and Pathology, Faculty of Medicine, Biomedical Sciences Group, KU Leuven, Herestraat 49, 3000 Leuven, Belgium. yicheng.ni@med.kuleuven.be |
| Key Words |
Cholelithiasis; Rat; Gallbladder; Common bile duct; Cholestasis; Bilirubin; Gallstones |
| Core Tip |
The mouse and rat are common experimental animals. Unlike the mouse that has a gallbladder but difficulty in imaging studies, the rat does not have a gallbladder, which has hampered studies on imaging gallstones. To tackle this problem, we first induced a virtual gallbladder (VGB) in rats by ligation of the common bile duct (CBD) accompanying with gradual increase and normalization of serum bilirubin. Then we implanted gallstone(s) collected from human patients into the dilated CBD or VGB to create a cholelithiatic model in rats, which has been validated by in vivo magnetic resonance imaging, microcholangiography and histology. This rat model is deemed useful for translational research such as fluorescent visualization of gallstones for laparoscopic detection and differential diagnosis of cholelithiasis. |
| Publish Date |
2016-06-23 18:00 |
| Citation |
Miranda Cona M, Liu Y, Yin T, Feng Y, Chen F, Mulier S, Li Y, Zhang J, Oyen R, Ni Y. Rat model of cholelithiasis with human gallstones implanted in cholestasis-induced virtual gallbladder. World J Methodol 2016; 6(2): 154-162 |
| URL |
http://www.wjgnet.com/2222-0682/full/v6/i2/154.htm |
| DOI |
http://dx.doi.org/10.5662/wjm.v6.i2.154 |
