Publication Name	World Journal of Stem Cells
Manuscript ID	58180
Country	Serbia

Received	2020-07-12 23:23
Peer-Review Started	2020-07-12 23:23
To Make the First Decision
Return for Revision	2020-09-24 19:35
Revised	2020-11-01 03:52
Second Decision	2020-11-17 09:48
Accepted by Journal Editor-in-Chief
Accepted by Company Editor-in-Chief	2020-11-17 22:49
Articles in Press	2020-11-17 22:49
Publication Fee Transferred
Edit the Manuscript by Language Editor	2020-11-24 23:43
Typeset the Manuscript	2021-01-14 01:39
Publish the Manuscript Online	2021-01-21 03:12

ISSN	1948-0210 (online)
Open Access	This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/Licenses/by-nc/4.0/
Copyright	The Author(s) 2020. Published by Baishideng Publishing Group Inc. All rights reserved.
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Category	Engineering, Biomedical
Manuscript Type	Basic Study
Article Title	Vascularization and osteogenesis in ectopically implanted bone tissue-engineered constructs with endothelial and osteogenic differentiated adipose-derived stem cells
Manuscript Source	Invited Manuscript
All Author List	Jelena G Najdanović, Vladimir J Cvetković, Sanja T Stojanović, Marija Đ Vukelić-Nikolić, Jelena M Živković and Stevo J Najman
Funding Agency and Grant Number	Funding Agency Grant Number Ministry of Education, Science and Technological Development of the Republic of Serbia III41017
Corresponding Author	Stevo J Najman, PhD, Full Professor, Department of Biology and Human Genetics; Department for Cell and Tissue Engineering, Faculty of Medicine, University of Niš, Blvd. Dr Zorana Djindjica 81, Niš 18108, Serbia. stevo.najman@medfak.ni.ac.rs
Key Words	Adipose-derived stem cells; Endothelial-related genes; Bone-related genes; Ectopic osteogenesis; Vascularization; Platelet-rich plasma
Core Tip	For successful bone tissue regeneration, osteogenesis and vasculogenesis should be supported by appropriate combination of cells, growth factors and biomaterials (biological triad). Since optimal triad factors composition is still unknown, our aim was to combine endothelial differentiated and osteogenic differentiated adipose-derived stem cells in the same construct together with platelet-rich plasma on bone mineral matrix as a carrier. Ectopic osteogenic potential of this construct was examined in mice subcutaneous implantation model which enables interplay between endothelial cells (ECs) and osteoblasts (OBs) in induction of ectopic osteogenesis. Obtained results unequivocally indicate the potential of such approach for further preclinical evaluations in orthotopic model regarding optimization of ECs/OBs ratio within bone tissue engineering constructs.
Publish Date	2021-01-21 03:12
Citation	Najdanović JG, Cvetković VJ, Stojanović ST, Vukelić-Nikolić MĐ, Živković JM, Najman SJ. Vascularization and osteogenesis in ectopically implanted bone tissue-engineered constructs with endothelial and osteogenic differentiated adipose-derived stem cells. World J Stem Cells 2021; 13(1): 91-114
URL	https://www.wjgnet.com/1948-0210/full/v13/i1/91.htm
DOI	https://dx.doi.org/10.4252/wjsc.v13.i1.91

Full Article (PDF)	WJSC-13-91.pdf
Full Article (Word)	WJSC-13-91.docx
The ARRIVE Guidelines	58180-The-ARRIVE-Guidelines-revision.pdf
Manuscript File	58180-Review-Filipodia.doc
Answering Reviewers	58180-Answering reviewers.pdf
Audio Core Tip	58180-Audio core tip.mp3
Biostatistics Review Certificate	58180-Biostatistics statement.pdf
Conflict-of-Interest Disclosure Form	58180-Conflict-of-interest statement.pdf
Copyright License Agreement	58180-Copyright license agreement.pdf
Approved Grant Application Form(s) or Funding Agency Copy of any Approval Document(s)	58180-Grant application form(s).pdf
Institutional Animal Care and Use Committee Approval Form or Document	58180-Institutional animal care and use committee statement.pdf
Institutional Review Board Approval Form or Document	58180-Institutional review board statement.pdf
Non-Native Speakers of English Editing Certificate	58180-Language certificate.pdf
Peer-review Report	58180-Peer-review(s).pdf
Scientific Misconduct Check	58180-Scientific misconduct check.pdf
Scientific Editor Work List	58180-Scientific editor work list.pdf