Publication Name	World Journal of Gastroenterology
Manuscript ID	75033
Country	Brazil

Received	2022-01-15 18:57
Peer-Review Started	2022-01-15 18:58
To Make the First Decision
Return for Revision	2022-02-07 08:35
Revised	2022-02-21 19:38
Second Decision	2022-05-06 02:56
Accepted by Journal Editor-in-Chief
Accepted by Company Editor-in-Chief	2022-05-07 00:18
Articles in Press	2022-05-07 00:18
Publication Fee Transferred
Edit the Manuscript by Language Editor
Typeset the Manuscript	2022-06-17 01:38
Publish the Manuscript Online	2022-06-24 13:12

ISSN	1007-9327 (print) and 2219-2840 (online)
Open Access	This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: https://creativecommons.org/Licenses/by-nc/4.0/
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Category	Genetics & Heredity
Manuscript Type	Basic Study
Article Title	Downregulation of TNFR2 decreases survival gene expression, promotes apoptosis and affects the cell cycle of gastric cancer cells
Manuscript Source	Invited Manuscript
All Author List	Ana Flávia Teixeira Rossi, Fernanda da Silva Manoel-Caetano, Joice Matos Biselli, Ágata Silva Cabral, Marilia de Freitas Calmon Saiki, Marcelo Lima Ribeiro and Ana Elizabete Silva
Funding Agency and Grant Number	Funding Agency Grant Number São Paulo Research Foundation (FAPESP) 2015/21464-0 São Paulo Research Foundation (FAPESP) 2015/23392-7 National Counsel of Technological and Scientific Development (CNPq) 310120/2015-2
Corresponding Author	Ana Elizabete Silva, PhD, Adjunct Associate Professor, Department of Biological Sciences, Sao Paulo State University (UNESP), Rua Cristóvão Colombo 2265, São José do Rio Preto 15054-000, São Paulo, Brazil. ae.silva@unesp.br
Key Words	Gastric cancer; Helicobacter pylori; Tumour necrosis factor-α signalling pathway; TNFR1; TNFR2; miRNAs
Core Tip	This study demonstrated for the first time the effect of TNFR1 and TNFR2 downregulation on an AGS cell line treated with Helicobacter pylori extract. Although TNFR1 downregulation did not promote significant changes in the expression of mRNA and miRNAs of the tumour necrosis factor-α (TNF-α) signalling pathway, TNFR2 downregulation promoted important changes in the signalling mediators evaluated. We observed a reduction in the expression of cell survival and anti-apoptotic genes and an increase in the expression of miR-19a and miR-34a, which affected cell cycle kinetics and contributed to early apoptosis. Thus, our findings highlight the important role of TNFR2 in the TNF-α signalling pathway in gastric carcinogenesis.
Publish Date	2022-06-24 13:12
Citation	Rossi AFT, da Silva Manoel-Caetano F, Biselli JM, Cabral AS, Saiki MFC, Ribeiro ML, Silva AE. Downregulation of TNFR2 decreases survival gene expression, promotes apoptosis and affects the cell cycle of gastric cancer cells. World J Gastroenterol 2022; 28(24): 2689-2704
URL	https://www.wjgnet.com/1007-9327/full/v28/i24/2689.htm
DOI	https://dx.doi.org/10.3748/wjg.v28.i24.2689

Full Article (PDF)	WJG-28-2689.pdf
Full Article (Word)	WJG-28-2689.docx
Manuscript File	75033_Auto_Edited.docx
Answering Reviewers	75033-Answering reviewers.pdf
Audio Core Tip	75033-Audio core tip.mp3
Biostatistics Review Certificate	75033-Biostatistics statement.pdf
Conflict-of-Interest Disclosure Form	75033-Conflict-of-interest statement.pdf
Copyright License Agreement	75033-Copyright license agreement.pdf
Approved Grant Application Form(s) or Funding Agency Copy of any Approval Document(s)	75033-Grant application form(s).pdf
Institutional Review Board Approval Form or Document	75033-Institutional review board statement.pdf
Non-Native Speakers of English Editing Certificate	75033-Language certificate.pdf
Supplementary Material	75033-Supplementary material.pdf
Peer-review Report	75033-Peer-review(s).pdf
Scientific Misconduct Check	75033-Bing-Fan JR-2.png
Scientific Editor Work List	75033-Scientific editor work list.pdf