ISSN |
1948-0210 (online) |
Open Access |
This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: https://creativecommons.org/Licenses/by-nc/4.0/ |
Copyright |
©The Author(s) 2023. Published by Baishideng Publishing Group Inc. All rights reserved. |
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Publisher |
Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA |
Website |
http://www.wjgnet.com |
Category |
Cell Biology |
Manuscript Type |
Basic Study |
Article Title |
Interferon-gamma and tumor necrosis factor-alpha synergistically enhance the immunosuppressive capacity of human umbilical-cord-derived mesenchymal stem cells by increasing PD-L1 expression
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Manuscript Source |
Unsolicited Manuscript |
All Author List |
Zhuo Chen, Meng-Wei Yao, Zhi-Lin Shen, Shi-Dan Li, Wei Xing, Wei Guo, Zhan Li, Xiao-Feng Wu, Luo-Quan Ao, Wen-Yong Lu, Qi-Zhou Lian, Xiang Xu and Xiang Ao |
Funding Agency and Grant Number |
Funding Agency |
Grant Number |
National Natural Science Foundation of China |
81871568, 32100643 |
COVID-19 Infection and Prevention Emergency Special Project of Chongqing Education Commission |
KYYJ202009 |
|
Corresponding Author |
Xiang Ao, PhD, Researcher, Department of Stem Cell & Regenerative Medicine, State Key Laboratory of Trauma, Burn and Combined Injury, Daping Hospital, Army Medical University, Changjiang Road, Yuzhong District, Chongqing 40042, China. ax_syd@163.com |
Key Words |
Human umbilical-cord-derived mesenchymal stem cells; Programmed cell death 1 ligand 1; Immunomodulation; Interferon-gamma; Tumor necrosis factor-alpha; Ulcerative colitis |
Core Tip |
Our study showed that interferon-gamma (IFN-γ) and tumor necrosis factor-alpha (TNF-α) significantly induced programmed cell death protein 1 ligand 1 (PD-L1) expression in human umbilical-cord-mesenchymal stem cells (hUC-MSCs), and pretreated hUC-MSCs exhibited stronger immunomodulatory capacity. Signaling pathway analysis showed that TNF-α up-regulated IFN-γ receptor expression in hUC-MSCs through nuclear factor kappa-B pathway, and then promoted IFN-γ -mediated activation of JAK/STAT1/interferon regulatory factor 1 pathway and PD-L1 expression. In ulcerative colitis mice, hUC-MSCs pretreated with IFN-γ and TNF-α exhibited stronger immunosuppressive ability through high expression of PD-L1, effectively inhibited the inflammation in the colon of mice. |
Publish Date |
2023-08-25 07:59 |
Citation |
Chen Z, Yao MW, Shen ZL, Li SD, Xing W, Guo W, Li Z, Wu XF, Ao LQ, Lu WY, Lian QZ, Xu X, Ao X. Interferon-gamma and tumor necrosis factor-alpha synergistically enhance the immunosuppressive capacity of human umbilical-cord-derived mesenchymal stem cells by increasing PD-L1 expression. World J Stem Cells 2023; 15(8): 787-806 |
URL |
https://www.wjgnet.com/1948-0210/full/v15/i8/787.htm |
DOI |
https://dx.doi.org/10.4252/wjsc.v15.i8.787 |