| ISSN |
1948-0210 (online) |
| Open Access |
This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/Licenses/by-nc/4.0/ |
| Copyright |
© The Author(s) 2024. Published by Baishideng Publishing Group Inc. All rights reserved. |
| Article Reprints |
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| Permissions |
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| Publisher |
Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA |
| Website |
http://www.wjgnet.com |
| Category |
Cell & Tissue Engineering |
| Manuscript Type |
Basic Study |
| Article Title |
Evaluation of genetic response of mesenchymal stem cells to nanosecond pulsed electric fields by whole transcriptome sequencing
|
| Manuscript Source |
Invited Manuscript |
| All Author List |
Jian-Jing Lin, Tong Ning, Shi-Cheng Jia, Ke-Jia Li, Yong-Can Huang, Qiang Liu, Jian-Hao Lin and Xin-Tao Zhang |
| Funding Agency and Grant Number |
| Funding Agency |
Grant Number |
| National Natural Science Foundation, China |
82272568, 81902247, 32201013 |
| Natural Science Foundation of Shandong Province, China |
ZR2021QH275 |
| Natural Science Foundation of Jinan City, China |
202225070 |
| Guangdong Basic and Applied Basic Research Foundation, China |
2022A1515220056 |
|
| Corresponding Author |
Xin-Tao Zhang, PhD, Chief Physician, Department of Sports Medicine and Rehabilitation, Peking University Shenzhen Hospital, No. 1120 Lianhua Road, Futian District, Shenzhen 518036, Guangdong Province, China. zhangxintao@sina.com |
| Key Words |
Nanosecond pulsed electric fields; Whole transcriptome sequencing; Mesenchymal stem cells; Genetic response; Stem cell engineering |
| Core Tip |
Nanosecond pulsed electric fields (nsPEFs) have been found to regulate the osteogenic, chondrogenic, and adipogenic differentiation of mesenchymal stem cells (MSCs). We hypothesized that several key factors may be regulated by nsPEFs, thereby influencing the biological functions of MSCs. Following exposure of MSCs to nsPEFs, we identified the differential expression of 263 messenger RNAs, 65 long noncoding RNAs, and 2 microRNAs. Verification by quantitative polymerase chain reaction and Gene Ontology and Kyoko Encyclopedia of Genes and Genomes enrichment analyses demonstrated the involvement of chromosome, cytoskeleton, and calcium signaling pathways following nsPEFs pretreatment. These results may be very meaningful for the further application of nsPEFs in MSCs. |
| Publish Date |
2024-03-25 02:43 |
| Citation |
Lin JJ, Ning T, Jia SC, Li KJ, Huang YC, Liu Q, Lin JH, Zhang XT. Evaluation of genetic response of mesenchymal stem cells to nanosecond pulsed electric fields by whole transcriptome sequencing. World J Stem Cells 2024; 16(3): 305-323 |
| URL |
https://www.wjgnet.com/1948-0210/full/v16/i3/305.htm |
| DOI |
https://dx.doi.org/10.4252/wjsc.v16.i3.305 |
