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3/25/2024 7:01:13 AM | Browse: 99 | Download: 163
Publication Name World Journal of Stem Cells
Manuscript ID 90791
Country China
2023-12-13 13:46
Peer-Review Started
2023-12-13 13:46
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Return for Revision
2024-01-24 06:04
2024-01-31 02:45
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2024-02-28 02:47
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2024-02-28 07:16
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2024-02-28 07:16
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Edit the Manuscript by Language Editor
2024-02-25 09:44
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2024-03-18 02:54
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2024-03-25 02:43
ISSN 1948-0210 (online)
Open Access This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/Licenses/by-nc/4.0/
Copyright © The Author(s) 2024. Published by Baishideng Publishing Group Inc. All rights reserved.
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Publisher Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA
Website http://www.wjgnet.com
Category Cell & Tissue Engineering
Manuscript Type Basic Study
Article Title Evaluation of genetic response of mesenchymal stem cells to nanosecond pulsed electric fields by whole transcriptome sequencing
Manuscript Source Invited Manuscript
All Author List Jian-Jing Lin, Tong Ning, Shi-Cheng Jia, Ke-Jia Li, Yong-Can Huang, Qiang Liu, Jian-Hao Lin and Xin-Tao Zhang
Funding Agency and Grant Number
Funding Agency Grant Number
National Natural Science Foundation, China 82272568, 81902247, 32201013
Natural Science Foundation of Shandong Province, China ZR2021QH275
Natural Science Foundation of Jinan City, China 202225070
Guangdong Basic and Applied Basic Research Foundation, China 2022A1515220056
Corresponding Author Xin-Tao Zhang, PhD, Chief Physician, Department of Sports Medicine and Rehabilitation, Peking University Shenzhen Hospital, No. 1120 Lianhua Road, Futian District, Shenzhen 518036, Guangdong Province, China. zhangxintao@sina.com
Key Words Nanosecond pulsed electric fields; Whole transcriptome sequencing; Mesenchymal stem cells; Genetic response; Stem cell engineering
Core Tip Nanosecond pulsed electric fields (nsPEFs) have been found to regulate the osteogenic, chondrogenic, and adipogenic differentiation of mesenchymal stem cells (MSCs). We hypothesized that several key factors may be regulated by nsPEFs, thereby influencing the biological functions of MSCs. Following exposure of MSCs to nsPEFs, we identified the differential expression of 263 messenger RNAs, 65 long noncoding RNAs, and 2 microRNAs. Verification by quantitative polymerase chain reaction and Gene Ontology and Kyoko Encyclopedia of Genes and Genomes enrichment analyses demonstrated the involvement of chromosome, cytoskeleton, and calcium signaling pathways following nsPEFs pretreatment. These results may be very meaningful for the further application of nsPEFs in MSCs.
Publish Date 2024-03-25 02:43
Citation Lin JJ, Ning T, Jia SC, Li KJ, Huang YC, Liu Q, Lin JH, Zhang XT. Evaluation of genetic response of mesenchymal stem cells to nanosecond pulsed electric fields by whole transcriptome sequencing. World J Stem Cells 2024; 16(3): 305-323
URL https://www.wjgnet.com/1948-0210/full/v16/i3/305.htm
DOI https://dx.doi.org/10.4252/wjsc.v16.i3.305
Full Article (PDF) WJSC-16-305-with-cover.pdf
The ARRIVE Guidelines 90791-The ARRIVE guidelines.pdf
Manuscript File 90791_Auto_Edited-JJW-WangTQ-YJP.docx
Answering Reviewers 90791-Answering reviewers.pdf
Audio Core Tip 90791-Audio core tip.m4a
Biostatistics Review Certificate 90791-Biostatistics statement.pdf
Conflict-of-Interest Disclosure Form 90791-Conflict-of-interest statement.pdf
Copyright License Agreement 90791-Copyright license agreement.pdf
Approved Grant Application Form(s) or Funding Agency Copy of any Approval Document(s) 90791-Grant application form(s).pdf
Institutional Animal Care and Use Committee Approval Form or Document 90791-Institutional animal care and use committee statement.pdf
Non-Native Speakers of English Editing Certificate 90791-Language certificate.pdf
Supplementary Material 90791-Supplementary material.pdf
Peer-review Report 90791-Peer-review(s).pdf
Scientific Misconduct Check 90791-Bing-Wang JJ-2.png
Scientific Editor Work List 90791-Scientific editor work list.pdf