Publication Name	World Journal of Gastroenterology
Manuscript ID	10656
Country	Germany

Received	2014-04-12 17:36
Peer-Review Started	2014-04-12 20:09
To Make the First Decision	2014-05-13 20:41
Return for Revision	2014-05-21 08:48
Revised	2014-06-15 00:00
Second Decision	2014-07-11 16:57
Accepted by Journal Editor-in-Chief
Accepted by Company Editor-in-Chief	2014-07-11 17:25
Articles in Press	2014-07-11 17:36
Publication Fee Transferred
Edit the Manuscript by Language Editor	2014-09-10 00:22
Typeset the Manuscript	2014-12-09 15:51
Publish the Manuscript Online	2014-12-20 19:42

ISSN	1007-9327 (print) and 2219-2840 (online)
Open Access
Copyright
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Publisher	Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA
Website	http://www.wjgnet.com

Category	Gastroenterology & Hepatology
Manuscript Type	Autobiography
Article Title	Elevated free cholesterol as a hallmark of non-alcoholic steatohepatitis in p62/insulin-like growth factor 2 mRNA binding protein 2-2 transgenic animals
Manuscript Source	Unsolicited Manuscript
All Author List	Yvette Simon, Sonja M Kessler, Katja Gemperlein, Rainer M Bohle, Rolf Müller, Johannes Haybaeck and Alexandra K Kiemer
Funding Agency and Grant Number	Funding Agency Grant Number An EASL Sheila Sherlock fellowship a Bank Austria visiting scientist program fellowship to Kessler SM
Corresponding Author	Alexandra K Kiemer, PhD, Department of Pharmacy, Saarland University, PO box 15 11 50, Saarbrücken 66041, Germany. pharm.bio.kiemer@mx.uni-saarland.de
Key Words	Insulin-like growth factor 2 mRNA binding protein 2-2; Methionine/choline deficient; Non-alcoholic fatty liver disease; Filipin; Iron
Core Tip	Dietary cholesterol represents a critical factor for the development from hepatic steatosis towards NASH. Liver-specific overexpression of the IGF2 mRNA binding protein p62/IMP2-2/IGF2BP2-2 induces steatosis and amplifies NASH-induced fibrosis. We here show that p62 elevates monounsaturated fatty acids as well as hepatic cholesterol in the absence of exogenous cholesterol. Filipin staining demonstrates increased free cholesterol in p62 transgenic livers. Srebf2-induced cholesterol biosynthesis in transgenics is most likely due to pronounced hepatic iron accumulation, which is also associated with lipid peroxidation in transgenic livers. In summary, our data show that p62/IGF2BP2-2 drives the progression of NASH by increasing hepatic free cholesterol.
Publish Date	2014-12-20 19:42
Citation	Simon Y, Kessler SM, Gemperlein K, Bohle RM, Müller R, Haybaeck J, Kiemer AK. Elevated free cholesterol in a p62 overexpression model of non-alcoholic steatohepatitis. World J Gastroenterol 2014; 20(47): 17839-17850
URL	http://www.wjgnet.com/1007-9327/full/v20/i47/17839.htm
DOI	http://dx.doi.org/10.3748/wjg.v20.i47.17839

Full Article (PDF)	WJG-20-17839.pdf
Full Article (Word)	WJG-20-17839.doc
Manuscript File	10656-Review.docx
Answering Reviewers	10656-Answering reviewers.pdf
Copyright License Agreement	10656-Copyright assignment.pdf
Peer-review Report	10656-Peer review.pdf
Scientific Misconduct Check	10656-CrossCheck.jpg
Scientific Editor Work List	10656-Scientific editor work list.pdf