Publication Name	World Journal of Virology
Manuscript ID	12055
Country	Thailand

Received	2014-06-20 20:06
Peer-Review Started	2014-06-20 21:57
To Make the First Decision	2014-08-14 15:02
Return for Revision	2014-08-19 13:16
Revised	2014-09-03 18:56
Second Decision	2014-10-16 15:06
Accepted by Journal Editor-in-Chief
Accepted by Executive Editor-in-Chief	2014-10-16 15:37
Articles in Press	2014-10-16 15:38
Publication Fee Transferred
Edit the Manuscript by Language Editor
Typeset the Manuscript	2014-11-13 17:23
Publish the Manuscript Online	2014-11-14 11:00

ISSN	2220-3249 (online)
Open Access
Copyright
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Website	http://www.wjgnet.com

Category	Virology
Manuscript Type	Autobiography
Article Title	Substrate specificity of avian influenza H5N1 neuraminidase
Manuscript Source	Unsolicited Manuscript
All Author List	Naruthai Onsirisakul, Shin-ichi Nakakita, Chompunuch Boonarkart, Alita Kongchanagul, Ornpreya Suptawiwat, Pilaipan Puthavathana, Krisada Chaichuen, Kanokwan Kittiniyom, Yasuo Suzuki and Prasert Auewarakul
Funding Agency and Grant Number	Funding Agency Grant Number Thailand Research Fund Office of the Higher Education Commission and Mahidol University under the National Research Universities Initiative
Corresponding Author	Prasert Auewarakul, Professor, Department of Microbiology, Faculty of Medicine Siriraj Hospital, Mahidol Univeristy, 2 Prannok Road, Bangkoknoi, Bangkok 10700, Thailand. sipaw@mahidol.ac.th
Key Words	H5N1 avian influenza virus; Neuraminidase; Sialic acid; Adaptation; Substrate preference
Core Tip	We analyzed neuraminidase (NA) substrate specificity of avian influenza H5N1 strains from humans and birds using a modified fluorescence assay, and the substrate specificity profile was further confirmed by high-performance liquid chromatography analysis of the enzymatic product. The H5N1 NA showed higher activity on ?2,3-linkage. Interestingly, NA from an H5N1 human isolate that was previously shown to have heamagglutinin (HA) with dual specificity showed reduced activity on ?2,3-linkage. These suggest that the H5N1 virus maintains the avian specific NA activity and that changes in the NA may be needed to compensate for changes in the HA specificity for the viral adaptation to human hosts.
Publish Date	2014-11-14 11:00
Citation	Sestak K. Role of histo-blood group antigens in primate enteric calicivirus infections. World J Virol 2014; 3(4): 30-36
URL	http://www.wjgnet.com/2220-3249/full/v3/i4/30.htm
DOI	http://dx.doi.org/10.5501/wjv.v3.i4.30

Full Article (PDF)	WJV-3-30.pdf
Full Article (Word)	WJV-3-30.doc
Manuscript File	12055-Review.docx
Answering Reviewers	12055-Answering reviewers.pdf
Copyright License Agreement	12055-Copyright asignment.pdf
Peer-review Report	12055-Peer review(s).pdf
Scientific Misconduct Check	12055-CrossCheck.jpg
Scientific Editor Work List	12055-Scientific editor work list.pdf