BPG is committed to discovery and dissemination of knowledge
Articles Published Processes
3/16/2015 6:04:00 PM | Browse: 1048 | Download: 1130
 |
Received |
|
2014-08-04 08:15 |
|
Peer-Review Started |
|
2014-08-04 11:01 |
|
To Make the First Decision |
|
2014-08-15 12:10 |
|
Return for Revision |
|
2014-08-19 15:44 |
|
Revised |
|
2014-09-09 23:44 |
|
Second Decision |
|
2014-11-19 08:42 |
 |
Accepted by Journal Editor-in-Chief |
|
|
|
Accepted by Executive Editor-in-Chief |
|
2014-12-01 13:38 |
|
Articles in Press |
|
2014-12-01 14:00 |
|
Publication Fee Transferred |
|
|
|
Edit the Manuscript by Language Editor |
|
2014-12-15 07:41 |
|
Typeset the Manuscript |
|
2015-03-03 16:55 |
|
Publish the Manuscript Online |
|
2015-03-16 18:04 |
| ISSN |
1007-9327 (print) and 2219-2840 (online) |
| Open Access |
This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/ |
| Copyright |
© The Author(s) 2015. Published by Baishideng Publishing Group Inc. All rights reserved. |
| Article Reprints |
For details, please visit: http://www.wjgnet.com/bpg/gerinfo/247
|
| Permissions |
For details, please visit: http://www.wjgnet.com/bpg/gerinfo/207
|
| Publisher |
Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA |
| Website |
http://www.wjgnet.com |
| Category |
Biochemistry & Molecular Biology |
| Manuscript Type |
Basic Study |
| Article Title |
Improved method increases sensitivity for circulating hepatocellular carcinoma cells
|
| Manuscript Source |
Unsolicited Manuscript |
| All Author List |
Hui-Ying Liu, Hai-Hua Qian, Xiao-Feng Zhang, Jun Li, Xia Yang, Bin Sun, Jun-Yong Ma, Lei Chen and Zheng-Feng Yin |
| Funding Agency and Grant Number |
| Funding Agency |
Grant Number |
| China National Key Projects for Infectious Disease |
2012ZX10002012-10 |
| National Nature Science Foundation of China |
81172207 |
| National Nature Science Foundation of China |
81272669 |
| National Nature Science Foundation of China |
81301830 |
|
| Corresponding Author |
Zheng-Feng Yin, MD, PhD, Molecular Oncology Laboratory, Eastern Hepatobiliary Surgery Hospital, Second Military Medical University, 225 Changhai Road, Shanghai 200438, China. yinzfk@aliyun.com |
| Key Words |
Asialoglycoprotein receptor; Carbamoyl phosphate synthetase 1; Circulating tumor cells; Hepatocellular carcinoma; Negative depletion |
| Core Tip |
We previously described asialoglycoprotein receptor (ASGPR)-based positive selection for the enrichment and further detection of circulating tumor cells (CTCs) of hepatocellular carcinoma (HCC). However, expression of ASGPR is heterogeneous in human HCC, and ASGPR- cells are missed by this method. In this study, we describe an improved method using depletion enrichment with identification using antibodies against ASGPR and carbamoyl phosphate synthetase 1. The improved method significantly improved sensitivity for CTC enrichment, and also provided high specificity for CTC detection in patients with HCC, thereby minimizing false negative/positive results. |
| Publish Date |
2015-03-16 18:04 |
| Citation |
Liu HY, Qian HH, Zhang XF, Li J, Yang X, Sun B, Ma JY, Chen L, Yin ZF. Improved method increases sensitivity for circulating hepatocellular carcinoma cells. World J Gastroenterol 2015; 21(10): 2918-2925 |
| URL |
http://www.wjgnet.com/1007-9327/full/v21/i10/2918.htm |
| DOI |
http://dx.doi.org/10.3748/wjg.v21.i10.2918 |
© 2004-2025 Baishideng Publishing Group Inc. All rights reserved. 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA
California Corporate Number: 3537345
