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3/16/2015 6:04:00 PM | Browse: 937 | Download: 892
Publication Name World Journal of Gastroenterology
Manuscript ID 13065
Country/Territory China
Received
2014-08-04 08:15
Peer-Review Started
2014-08-04 11:01
To Make the First Decision
2014-08-15 12:10
Return for Revision
2014-08-19 15:44
Revised
2014-09-09 23:44
Second Decision
2014-11-19 08:42
Accepted by Journal Editor-in-Chief
Accepted by Company Editor-in-Chief
2014-12-01 13:38
Articles in Press
2014-12-01 14:00
Publication Fee Transferred
Edit the Manuscript by Language Editor
2014-12-15 07:41
Typeset the Manuscript
2015-03-03 16:55
Publish the Manuscript Online
2015-03-16 18:04
ISSN 1007-9327 (print) and 2219-2840 (online)
Open Access This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
Copyright © The Author(s) 2015. Published by Baishideng Publishing Group Inc. All rights reserved.
Article Reprints For details, please visit: http://www.wjgnet.com/bpg/gerinfo/247
Permissions For details, please visit: http://www.wjgnet.com/bpg/gerinfo/207
Publisher Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA
Website http://www.wjgnet.com
Category Biochemistry & Molecular Biology
Manuscript Type Basic Study
Article Title Improved method increases sensitivity for circulating hepatocellular carcinoma cells
Manuscript Source Unsolicited Manuscript
All Author List Hui-Ying Liu, Hai-Hua Qian, Xiao-Feng Zhang, Jun Li, Xia Yang, Bin Sun, Jun-Yong Ma, Lei Chen and Zheng-Feng Yin
Funding Agency and Grant Number
Funding Agency Grant Number
China National Key Projects for Infectious Disease 2012ZX10002012-10
National Nature Science Foundation of China 81172207
National Nature Science Foundation of China 81272669
National Nature Science Foundation of China 81301830
Corresponding Author Zheng-Feng Yin, MD, PhD, Molecular Oncology Laboratory, Eastern Hepatobiliary Surgery Hospital, Second Military Medical University, 225 Changhai Road, Shanghai 200438, China. yinzfk@aliyun.com
Key Words Asialoglycoprotein receptor; Carbamoyl phosphate synthetase 1; Circulating tumor cells; Hepatocellular carcinoma; Negative depletion
Core Tip We previously described asialoglycoprotein receptor (ASGPR)-based positive selection for the enrichment and further detection of circulating tumor cells (CTCs) of hepatocellular carcinoma (HCC). However, expression of ASGPR is heterogeneous in human HCC, and ASGPR- cells are missed by this method. In this study, we describe an improved method using depletion enrichment with identification using antibodies against ASGPR and carbamoyl phosphate synthetase 1. The improved method significantly improved sensitivity for CTC enrichment, and also provided high specificity for CTC detection in patients with HCC, thereby minimizing false negative/positive results.
Publish Date 2015-03-16 18:04
Citation Liu HY, Qian HH, Zhang XF, Li J, Yang X, Sun B, Ma JY, Chen L, Yin ZF. Improved method increases sensitivity for circulating hepatocellular carcinoma cells. World J Gastroenterol 2015; 21(10): 2918-2925
URL http://www.wjgnet.com/1007-9327/full/v21/i10/2918.htm
DOI http://dx.doi.org/10.3748/wjg.v21.i10.2918
Full Article (PDF) WJG-21-2918.pdf
Full Article (Word) WJG-21-2918.doc
Manuscript File 13065-Review.docx
Answering Reviewers 13065-Answering reviewers.pdf
Copyright License Agreement 13065-Copyright assignment.pdf
Non-Native Speakers of English Editing Certificate 13065-Language certificate.pdf
Peer-review Report 13065-Peer reviews.pdf
Scientific Editor Work List 13065-Scientific editor work list.pdf