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Articles Published Processes
8/28/2015 5:05:00 PM | Browse: 1250 | Download: 2216
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Received |
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2015-02-21 10:13 |
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Peer-Review Started |
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2015-02-22 20:16 |
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To Make the First Decision |
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2015-04-23 13:14 |
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Return for Revision |
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2015-04-29 18:08 |
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Revised |
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2015-05-20 15:15 |
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Second Decision |
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2015-07-03 20:51 |
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Accepted by Journal Editor-in-Chief |
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Accepted by Executive Editor-in-Chief |
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2015-07-08 17:13 |
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Articles in Press |
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2015-07-08 17:13 |
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Publication Fee Transferred |
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Edit the Manuscript by Language Editor |
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2015-07-21 21:55 |
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Typeset the Manuscript |
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2015-08-12 17:31 |
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Publish the Manuscript Online |
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2015-08-28 17:04 |
| ISSN |
1007-9327 (print) and 2219-2840 (online) |
| Open Access |
This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/ |
| Copyright |
© The Author(s) 2015. Published by Baishideng Publishing Group Inc. All rights reserved.
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| Article Reprints |
For details, please visit: http://www.wjgnet.com/bpg/gerinfo/247
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| Permissions |
For details, please visit: http://www.wjgnet.com/bpg/gerinfo/207
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| Publisher |
Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA |
| Website |
http://www.wjgnet.com |
| Category |
Virology |
| Manuscript Type |
Basic Study |
| Article Title |
Dual gRNAs guided CRISPR/Cas9 system inhibits hepatitis B virus replication
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| Manuscript Source |
Unsolicited Manuscript |
| All Author List |
Jie Wang, Zhong-Wei Xu, Shuang Liu, Rui-Yang Zhang, Shan-Long Ding, Xiao-Meng Xie, Lu Long, Xiang-Mei Chen, Hui Zhuang and Feng-Min Lu |
| Funding Agency and Grant Number |
| Funding Agency |
Grant Number |
| Natural Science Foundation of China |
81471938 |
| National S and T Major Project for Infectious Diseases |
2013ZX10002-002 |
| National S and T Major Project for Infectious Diseases |
2012ZX10002-005 |
| 111 Project |
B07001 |
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| Corresponding Author |
Feng-Min Lu, MD, Professor, State Key Laboratory of Natural and Biomimetic Drugs, Department of Microbiology and Infectious Disease Center, School of Basic Medicine, Peking University Health Science Center, 38 Xueyuan Road, Beijing 100191, China. lu.fengmin@hsc.pku.edu.cn |
| Key Words |
Dual gRNAs; CRISPR/Cas9; hepatitis B; cccDNA; antiviral therapy |
| Core Tip |
In this manuscript, 15 hepatitis B virus (HBV)-specific gRNAs were designed according to the HBV genome sequences of genotypes A-D. We confirmed that the CRISPR/Cas9 system with these HBV-specific gRNAs could efficiently suppress the replication of multiple HBV genotypes. Further, we demonstrated that dual gRNAs could guide the CRISPR/Cas9 system to efficiently destroy HBV cccDNA and reduce its level in HepAD38 cells. Since cccDNA, the template of HBV replication, accounts for the persistence of HBV infection, our data suggested that CRISPR/Cas9 technique may be a useful tool to eradicate HBV of multiple genotypes. |
| Publish Date |
2015-08-28 17:04 |
| Citation |
Wang J, Xu ZW, Liu S, Zhang RY, Ding SL, Xie XM, Long L, Chen XM, Zhuang H, Lu FM. Dual gRNAs guided CRISPR/Cas9 system inhibits hepatitis B virus replication. World J Gastroenterol 2015; 21(32): 9554-9565 |
| URL |
http://www.wjgnet.com/1007-9327/full/v21/i32/9554.htm |
| DOI |
http://dx.doi.org/10.3748/wjg.v21.i32.9554 |
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