Publication Name	World Journal of Gastroenterology
Manuscript ID	24264
Country	Italy

Received	2016-01-21 11:55
Peer-Review Started	2016-01-21 16:49
To Make the First Decision	2016-02-18 11:48
Return for Revision	2016-02-19 11:19
Revised	2016-03-02 18:38
Second Decision	2016-03-04 18:03
Accepted by Journal Editor-in-Chief	2016-03-05 15:54
Accepted by Company Editor-in-Chief	2016-03-14 10:22
Articles in Press	2016-03-14 10:22
Publication Fee Transferred
Edit the Manuscript by Language Editor	2016-03-28 01:59
Typeset the Manuscript	2016-04-07 09:10
Publish the Manuscript Online	2016-05-04 10:08

ISSN	1007-9327 (print) and 2219-2840 (online)
Open Access	This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/ Peer-review started: February 25, 2016
Copyright	© The Author(s) 2016. Published by Baishideng Publishing Group Inc. All rights reserved.
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Category	Cell Biology
Manuscript Type	Basic Study
Article Title	Epithelial-to-mesenchymal transition in pancreatic ductal adenocarcinoma: Characterization in a 3D-cell culture model
Manuscript Source	Invited Manuscript
All Author List	Nicoletta Gagliano, Giuseppe Celesti, Lorenza Tacchini, Stefano Pluchino, Chiarella Sforza, Marco Rasile, Vincenza Valerio, Luigi Laghi, Vincenzo Conte and Patrizia Procacci
Funding Agency and Grant Number	Funding Agency Grant Number University of Milan (Project B grant)
Corresponding Author	Nicoletta Gagliano, PhD, Associate Professor of Histology, Department of Biomedical Sciences for Health, Università degli Studi di Milano, via Mangiagalli 31, 20133 Milan, Italy. nicoletta.gagliano@unimi.it
Key Words	Epithelial-to-mesenchymal transition; E-cadherin; 3D-spheroids; Podoplanin; Pancreatic ductal adenocarcinoma
Core Tip	The functions of living tissue can be mimicked by three-dimensional (3D) cell cultures, thereby providing a method of decoding the information encoded in the tissue architecture. We aimed to analyze the effect of 3D-arrangement on the expression of some key markers of epithelial-to-mesenchymal transition in pancreatic adenocarcinoma (PDAC) cells cultured in either 2D-monolayers or 3D-spheroids. Our results show that a 3D-cell culture model could provide deeper insight into understanding the biology of PDAC and allow for the detection of marked differences in the phenotype of PDAC cells grown in 3D-spheroids.
Publish Date	2016-05-04 10:08
Citation	Gagliano N, Celesti G, Tacchini L, Pluchino S, Sforza C, Rasile M, Valerio V, Laghi L, Conte V, Procacci P. Epithelial-to-mesenchymal transition in pancreatic ductal adenocarcinoma: Characterization in a 3D-cell culture model. World J Gastroenterol 2016; 22(18): 4466-4483
URL	http://www.wjgnet.com/1007-9327/full/v22/i18/4466.htm
DOI	http://dx.doi.org/10.3748/wjg.v22.i18.4466

Full Article (PDF)	WJG-22-4466.pdf
Full Article (Word)	WJG-22-4466.doc
Manuscript File	24264-Review.docx
Answering Reviewers	2464-Answering reviewers.pdf
Audio Core Tip	24264-Audio core tip.mp3
Biostatistics Review Certificate	24264-Biostatistics statement.pdf
Conflict-of-Interest Disclosure Form	24264-Conflict-of-interest statement.pdf
Copyright License Agreement	24264-Copyright assignment.pdf
Approved Grant Application Form(s) or Funding Agency Copy of any Approval Document(s)	24264-Grant application form(s).pdf
Institutional Animal Care and Use Committee Approval Form or Document	24264-Institutional animal care and use committee statement.pdf
Institutional Review Board Approval Form or Document	24264-Institutional review board statement.pdf
Non-Native Speakers of English Editing Certificate	24264-Language certificate.pdf
Peer-review Report	24264-Peer-review(s).pdf
Journal Editor-in-Chief Review Report	24264-Journal editor-in-chief review report.pdf
Scientific Misconduct Check	24264-Scientific misconduct check.pdf
Scientific Editor Work List	24264-Scientific editor work list.pdf