| ISSN |
1007-9327 (print) and 2219-2840 (online) |
| Open Access |
This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
|
| Copyright |
© The Author(s) 2016. Published by Baishideng Publishing Group Inc. All rights reserved.
|
| Article Reprints |
For details, please visit: http://www.wjgnet.com/bpg/gerinfo/247
|
| Permissions |
For details, please visit: http://www.wjgnet.com/bpg/gerinfo/207
|
| Publisher |
Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA |
| Website |
http://www.wjgnet.com |
| Category |
Infectious Diseases |
| Manuscript Type |
Observational Study |
| Article Title |
New real-time-PCR method to identify single point mutations in hepatitis C virus
|
| Manuscript Source |
Invited Manuscript |
| All Author List |
Qian Chen, Irene Belmonte, Maria Buti, Leonardo Nieto, Damir Garcia-Cehic, Josep Gregori, Celia Perales, Laura Ordeig, Meritxell Llorens, Maria Eugenia Soria, Rafael Esteban, Juan Ignacio Esteban, Francisco Rodriguez-Frias and Josep Quer |
| Funding Agency and Grant Number |
| Funding Agency |
Grant Number |
| Instituto de Salud Carlos III/ERDF |
PI12/01893 |
| Instituto de Salud Carlos III/ERDF |
PI13/00456 |
| Instituto de Salud Carlos III/ERDF |
PI15/00829 |
| Instituto de Salud Carlos III/ERDF |
PI15/00856 |
| Miguel Servet program of the Instituto de Salud Carlos III |
CP14/00121 |
| Gilead |
GLD14/00296 |
| CIBERehd (Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas) is funded by Instituto de Salud Carlos III |
|
|
| Corresponding Author |
Francisco Rodriguez-Frias, PhD, Liver Pathology Unit, Departments of Biochemistry and Microbiology, Hospital Universitari Vall d’Hebron, Autonomous University of Barcelona, Pg Vall d’Hebron 119-129, 08035 Barcelona,
Spain. frarodri@vhebron.net
|
| Key Words |
Hepatitis C virus; Resistance-associated amino acid substitutions; Low-cost test; Single-point mutations; Q80K |
| Core Tip |
This report describes a new low-cost, fast and highly sensitive technique based on real-time PCR and specific fluorescence resonance energy transfer probe melting curve analysis to identify single-point mutations in a highly variable background (in this study the Q80K resistance-associated mutation in hepatitis C virus - infected patients) for routine use in clinical laboratories. This technique can be adapted to detect any mutation in a highly variable genome.
|
| Publish Date |
2016-11-18 15:13 |
| Citation |
Chen Q, Belmonte I, Buti M, Nieto L, Garcia-Cehic D, Gregori J, Perales C, Ordeig L, Llorens M, Soria ME, Esteban R, Esteban JI, Rodriguez-Frias F, Quer J. New real-time-PCR method to identify single point mutations in hepatitis C virus. World J Gastroenterol 2016; 22(43): 9604-9612 |
| URL |
http://www.wjgnet.com/1007-9327/full/v22/i43/9604.htm |
| DOI |
http://dx.doi.org/10.3748/wjg.v22.i43.9604 |
