Publication Name	World Journal of Gastroenterology
Manuscript ID	39776
Country	China

Received	2018-05-11 06:08
Peer-Review Started	2018-05-11 08:07
To Make the First Decision	2018-06-06 02:08
Return for Revision	2018-06-11 08:06
Revised	2018-06-17 08:28
Second Decision	2018-06-28 08:09
Accepted by Journal Editor-in-Chief
Accepted by Executive Editor-in-Chief	2018-06-28 16:20
Articles in Press	2018-06-28 16:20
Publication Fee Transferred
Edit the Manuscript by Language Editor	2018-07-07 17:35
Typeset the Manuscript	2018-08-08 03:11
Publish the Manuscript Online	2018-08-13 07:45

ISSN	1007-9327 (print) and 2219-2840 (online)
Open Access	This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
Copyright	© The Author(s) 2018. Published by Baishideng Publishing Group Inc. All rights reserved.
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Category	Engineering, Biomedical
Manuscript Type	Basic Study
Article Title	Novel sericin-based hepatocyte serum-free medium and sericin’s effect on hepatocyte transcriptome
Manuscript Source	Unsolicited Manuscript
All Author List	Yun Huang, Qing Peng, Hai-Yan Li, Zhi-Dong Jia, Yang Li and Yi Gao
ORCID	Author(s) ORCID Number Yun Huang http://orcid.org/0000-0002-4247-8964 Qing Peng http://orcid.org/0000-0002-7790-6455 Hai-Yan Li http://orcid.org/0000-0002-3842-8964 Zhi-Dong Jia http://orcid.org/0000-0001-5362-5205 Yang Li http://orcid.org/0000-0003-4718-1825 Yi Gao http://orcid.org/0000-0003-3525-0133
Funding Agency and Grant Number	Funding Agency Grant Number The National Natural Science Foundation of China 81470875 The Natural Science Foundation of Guangdong Province 2014A030312013 Science and Technology Planning Project of Guangdong Province 2014B020227002 Science and Technology Planning Project of Guangdong Province 2015B090903069 Science and Technology Planning Project of Guangdong Province 2015B020229002 Science and Technology Program of Guangzhou 201604020002
Corresponding Author	Yi Gao, MD, PhD, Chief Doctor, Professor, Surgeon, Department of Hepatobiliary Surgery II, Guangdong Provincial Research Center for Artificial Organ and Tissue Engineering, Guangzhou Clinical Research and Transformation Center for Artificial Liver, Institute of Regenerative Medicine,, Zhujiang Hospital of Southern Medical University, 253 Middle Industrial Avenue, Guangzhou 510280, Guangdong Province, China. gaoyi6146@163.com
Key Words	Sericin; Serum-free medium; MAPK pathway; Bioartificial liver support system; CCR6
Core Tip	In recent decades, few studies have focused on the development of hepatocyte serum-free medium. In this study, we developed a novel hepatocyte serum-free medium suitable for in vitro culture of C3A cells and applied an advanced method, gene-chip array, to explore the effect of sericin on the hepatocyte transcriptome. We found that sericin probably enhanced cell attachment through the CCR6-Akt-JNK-NF-κB pathway and promoted cell proliferation through CCR6-mediated activation of the ERK1/2-MAPK pathway. These findings inspired the fol­lowing study on the mechanism by which sericin promotes cell attachment and proliferation.
Publish Date	2018-08-13 07:45
Citation	Huang Y, Peng Q, Li HY, Jia ZD, Li Y, Gao Y. novel sericin-based hepatocyte serum-free medium and sericin’s effect on hepatocyte transcriptome. World J Gastroenterol 2018; 24(30): 3398-3413
URL	http://www.wjgnet.com/1007-9327/full/v24/i30/3398.htm
DOI	http://dx.doi.org/10.3748/wjg.v24.i30.3398

Full Article (PDF)	WJG-24-3398.pdf
Manuscript File	39776-Review.docx
Answering Reviewers	39776-Answering reviewers.pdf
Audio Core Tip	39776-Audio core tip.mp3
Biostatistics Review Certificate	39776-Biostatistics statement.pdf
Conflict-of-Interest Disclosure Form	39776-Conflict-of-interest statement.pdf
Copyright License Agreement	39776-Copyright license agreement.pdf
Approved Grant Application Form(s) or Funding Agency Copy of any Approval Document(s)	39776-Grant application form(s).pdf
Non-Native Speakers of English Editing Certificate	39776-Language certificate.pdf
Peer-review Report	39776-Peer-review(s).pdf
Scientific Misconduct Check	39776-Scientific misconduct check.pdf
Scientific Editor Work List	39776-Scientific editor work list.pdf