| ISSN |
1948-0210 (online) |
| Open Access |
This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/ |
| Copyright |
© The Author(s) 2019. Published by Baishideng Publishing Group Inc. All rights reserved. |
| Article Reprints |
For details, please visit: http://www.wjgnet.com/bpg/gerinfo/247
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| Permissions |
For details, please visit: http://www.wjgnet.com/bpg/gerinfo/207
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| Publisher |
Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA |
| Website |
http://www.wjgnet.com |
| Category |
Neurosciences |
| Manuscript Type |
Basic Study |
| Article Title |
Triple-modal imaging of stem-cells labeled with multimodal nanoparticles, applied in a stroke model
|
| Manuscript Source |
Unsolicited Manuscript |
| All Author List |
Helio Rodrigues da Silva, Javier Bustamante Mamani, Mariana Penteado Nucci, Leopoldo Penteado Nucci, Andrea Tiemi Kondo, Daianne Maciely Carvalho Fantacini, Lucas Eduardo Botelho de Souza, Virginia Picanço-Castro, Dimas Tadeu Maciely Covas, José Mauro Kutner, Fernando Anselmo de Oliveira, Nelson Hamerschlak and Lionel Fernel Gamarra |
| ORCID |
|
| Funding Agency and Grant Number |
| Funding Agency |
Grant Number |
| Conselho Nacional de Desenvolvimento Científico e Tecnológico |
CNPq-465259/2014-6 |
| Conselho Nacional de Desenvolvimento Científico e Tecnológico |
CNPq-400856/2016-6 |
| São Paulo State Research Support Foundation |
2014/50983-3 |
| São Paulo State Research Support Foundation |
2016/21470-3 |
|
| Corresponding Author |
Lionel Fernel Gamarra, MSc, PhD, Academic Research, Associate Research Scientist, Postdoc, Professor, Reader in Health Technology Assessment, Research Scientist, Senior Researcher, Statistician, Group of nanobiotechnology of The Albert Einstein Hospital,, Hospital Israelita Albert Einstein, Avenida Albert Einstein, 627/701 – Morumbi, São Paulo 05651-901, Brazil. lionelgamarra7@gmail.com |
| Key Words |
Multimodal nanoparticles; Human bone marrow mesenchymal stem cells; Near-infrared fluorescence image; Magnetic resonance image; Bioluminescence; Stroke |
| Core Tip |
Multimodal imaging techniques provide morpho-functional information for studying pathological events following ischemia associated with new tracers. Molecular imaging innovations will contribute to the further understanding of stem cell transplantation, allowing an assessment of their therapeutic effects at the molecular scale. In this study, we evaluate the sensitivity of triple-modal imaging of human bone marrow mesenchymal stem cells labeled with multimodal nanoparticles (MNPs) to quantify the internalized iron load and cellular viability as well as the correlation of quantification results between the techniques. We demonstrate the importance of quantifying the MNP load internalized into cells via triple-image evaluation and the efficacy of signal detection in a stroke model. |
| Publish Date |
2019-02-26 03:18 |
| Citation |
da Silva HR, Mamani JB, Nucci MP, Nucci LP, Kondo AT, Fantacini DMC, de Souza LEB, Picanço-Castro V, Covas DT, Kutner JM, de Oliveira FA, Hamerschlak N, Gamarra LF. Triple-modal imaging of stem-cells labeled with multimodal nanoparticles, applied in a stroke model. World J Stem Cells 2019; 11(2): 100-123 |
| URL |
https://www.wjgnet.com/1948-0210/full/v11/i2/100.htm |
| DOI |
https://dx.doi.org/10.4252/wjsc.v11.i2.100 |
