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9/30/2019 2:18:31 AM | Browse: 32 | Download: 43
Publication Name World Journal of Clinical Oncology
Manuscript ID 47274
Country of Manuscript Source Romania
Received
2019-03-12 03:37
Peer-Review Started
2019-03-20 04:15
To Make the First Decision
2019-04-16 07:47
Return for Revision
2019-05-13 02:49
Revised
2019-08-19 08:54
Second Decision
2019-08-29 08:57
Accepted by Journal Editor-in-Chief
Accepted by Company Editor-in-Chief
2019-09-05 17:39
Articles in Press
2019-09-05 17:39
Publication Fee Transferred
Edit the Manuscript by Language Editor
Typeset the Manuscript
2019-09-17 03:38
Publish the Manuscript Online
2019-09-30 02:18
ISSN 2218-4333 (online)
Open Access This is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
Copyright © The Author(s) 2019. Published by Baishideng Publishing Group Inc. All rights reserved.
Article Reprints For details, please visit: http://www.wjgnet.com/bpg/gerinfo/247
Permissions For details, please visit: http://www.wjgnet.com/bpg/gerinfo/207
Publisher Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA
Website http://www.wjgnet.com
Specialty Oncology
Manuscript type Observational Study
Article Title DNA extraction from paraffin embedded colorectal carcinoma samples: A comparison study of manual vs automated methods, using four commercially kits
Manuscript Source Invited Manuscript
All Author List Zsolt Kovacs, Ioan Jung, Erzsebet Csernak, Zoltan Szentirmay, Laura Banias, Genoveva Rigmanyi and Simona Gurzu
ORCID
Author(s) ORCID Number
Zsolt Kovacs http://orcid.org/0000-0002-1038-7769
Ioan Jung http://orcid.org/0000-0001-6537-2807
Erzsebet Csernak http://orcid.org/0000-0002-4311-5364
Zoltan Szentirmay http://orcid.org/0000-0003-1624-5398
Laura Banias http://orcid.org/0000-0002-2240-2540
Genoveva Rigmanyi http://orcid.org/0000-0001-9093-6598
Simona Gurzu http://orcid.org/0000-0003-3968-5118
Funding Agency and Grant Number
Correspondence To Simona Gurzu, MD, PhD, Chief Doctor, Director, Doctor, Full Professor, Research Fellow, Research Scientist, Professor, Consulting Pathologist, Pathology, University of Medicine, Pharmacy, Sciences and Technology, Ghe marinescu 38, Tirgu-Mures 540139, Non-US/Non-Canadian, Romania. simonagurzu@yahoo.com
Keywords DNA isolation; Colorectal cancer; Paraffin-embedded; PureLink Genomic DNA Mini Kit; High Pure FFPE DNA Isolation Kit; iPrep Genomic DNA Kit
Core Tip In this paper we proved the advantages and disadvantages of manual vs automated methods of DNA extraction. The original aspect refers to the correlation between DNA concentration and colorectal adenocarcinoma (CRC) features. We conclude that manual methods are more suitable for obtaining high DNA concentrations, especially from differentiated polypoid-type CRCs. In CRC samples, a higher DNA concentration is associated with a lower OD value.
Publish Date 2019-09-30 02:18
Citation Kovacs Z, Jung I, Csernak E, Szentirmay Z, Banias L, Rigmanyi G, Gurzu S. DNA extraction from paraffin embedded colorectal carcinoma samples: A comparison study of manual vs automated methods, using four commercially kits. World J Clinical Oncol 2019; 10(9): 307-317
Url https://www.wjgnet.com/2218-4333/full/v10/i9/307.htm
DOI https://dx.doi.org/10.5306/wjco.v10.i9.307
Full Article (PDF) WJCO-10-307.pdf
Full Article (Word) WJCO-10-307.docx
Manuscript File 47274-Review.doc
Answering Reviewers 47274-Answering reviewers.pdf
Audio Core Tip 47274-Audio core tip.m4a
Biostatistics Review Certificate 47274-Biostatistics statement.pdf
Conflict-of-Interest Disclosure Form 47274-Conflict-of-interest statement.pdf
Copyright License Agreement 47274-Copyright license agreement.pdf
Approved Grant Application Form(s) or Funding Agency Copy of any Approval Document(s) 47274-Grant application form(s).pdf
Institutional Review Board Approval Form or Document 47274-Institutional review board statement.pdf
Non-Native Speakers of English Editing Certificate 47274-Language certificate.pdf
Peer-review Report 47274-Peer-review(s).pdf
Scientific Misconduct Check 47274-Bing-Tang JZ-2.png
Scientific Misconduct Check 47274-Scientific misconduct check.pdf
Scientific Editor Work List 47274-Scientific editor work list.pdf