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Articles Published Processes
9/30/2019 2:18:31 AM | Browse: 699 | Download: 960
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Received |
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2019-03-12 03:37 |
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Peer-Review Started |
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2019-03-20 04:15 |
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To Make the First Decision |
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2019-04-16 07:47 |
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Return for Revision |
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2019-05-13 02:49 |
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Revised |
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2019-08-19 08:54 |
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Second Decision |
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2019-08-29 08:57 |
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Accepted by Journal Editor-in-Chief |
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Accepted by Company Editor-in-Chief |
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2019-09-05 17:39 |
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Articles in Press |
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2019-09-05 17:39 |
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Publication Fee Transferred |
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Edit the Manuscript by Language Editor |
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Typeset the Manuscript |
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2019-09-17 03:38 |
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Publish the Manuscript Online |
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2019-09-30 02:18 |
| ISSN |
2218-4333 (online) |
| Open Access |
This is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/ |
| Copyright |
© The Author(s) 2019. Published by Baishideng Publishing Group Inc. All rights reserved. |
| Article Reprints |
For details, please visit: http://www.wjgnet.com/bpg/gerinfo/247
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| Permissions |
For details, please visit: http://www.wjgnet.com/bpg/gerinfo/207
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| Publisher |
Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA |
| Website |
http://www.wjgnet.com |
| Category |
Oncology |
| Manuscript Type |
Observational Study |
| Article Title |
DNA extraction from paraffin embedded colorectal carcinoma samples: A comparison study of manual vs automated methods, using four commercially kits
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| Manuscript Source |
Invited Manuscript |
| All Author List |
Zsolt Kovacs, Ioan Jung, Erzsebet Csernak, Zoltan Szentirmay, Laura Banias, Genoveva Rigmanyi and Simona Gurzu |
| ORCID |
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| Funding Agency and Grant Number |
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| Corresponding Author |
Simona Gurzu, MD, PhD, Chief Doctor, Director, Doctor, Full Professor, Research Fellow, Research Scientist, Pathology, University of Medicine, Pharmacy, Sciences and Technology, Ghe marinescu 38, Tirgu-Mures 540139, Non-US/Non-Canadian, Romania. simonagurzu@yahoo.com |
| Key Words |
DNA isolation; Colorectal cancer; Paraffin-embedded; PureLink Genomic DNA Mini Kit; High Pure FFPE DNA Isolation Kit; iPrep Genomic DNA Kit |
| Core Tip |
In this paper we proved the advantages and disadvantages of manual vs automated methods of DNA extraction. The original aspect refers to the correlation between DNA concentration and colorectal adenocarcinoma (CRC) features. We conclude that manual methods are more suitable for obtaining high DNA concentrations, especially from differentiated polypoid-type CRCs. In CRC samples, a higher DNA concentration is associated with a lower OD value. |
| Publish Date |
2019-09-30 02:18 |
| Citation |
Kovacs Z, Jung I, Csernak E, Szentirmay Z, Banias L, Rigmanyi G, Gurzu S. DNA extraction from paraffin embedded colorectal carcinoma samples: A comparison study of manual vs automated methods, using four commercially kits. World J Clinical Oncol 2019; 10(9): 307-317 |
| URL |
https://www.wjgnet.com/2218-4333/full/v10/i9/307.htm |
| DOI |
https://dx.doi.org/10.5306/wjco.v10.i9.307 |
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