| ISSN |
1948-5182 (online) |
| Open Access |
This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: https://creativecommons.org/Licenses/by-nc/4.0/ |
| Copyright |
© The Author(s) 2022. Published by Baishideng Publishing Group Inc. All rights reserved. |
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| Permissions |
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| Publisher |
Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA |
| Website |
http://www.wjgnet.com |
| Category |
Transplantation |
| Manuscript Type |
Basic Study |
| Article Title |
Long-term and non-invasive in vivo tracking of DiD-labeled human hepatic progenitors in chronic liver disease models
|
| Manuscript Source |
Unsolicited Manuscript |
| All Author List |
Chaturvedula Tripura, Srinivas Gunda, Sandeep Kumar Vishwakarma, Avinash Raj Thatipalli, Jedy Jose, Mahesh Kumar Jerald, Aleem Ahmed Khan and Gopal Pande |
| ORCID |
|
| Funding Agency and Grant Number |
| Funding Agency |
Grant Number |
| Department of Science and Technology (DST), Ministry of Science and Technology, Govt. of India and Indian Council of Medical Research (ICMR), New Delhi, Govt. of India Grants to GP |
GAP-0220 |
| Department of Science and Technology (DST), Ministry of Science and Technology, Govt. of India and Indian Council of Medical Research (ICMR), New Delhi, Govt. of India Grants to GP |
GAP-0383 |
|
| Corresponding Author |
Chaturvedula Tripura, PhD, Senior Scientist, Cell and Stem Cell Biology, CSIR-Centre for Cellular and Molecular Biology, Uppal Road, Habsiguda, Hyderabad 500007, Telangana, India. tripura@ccmb.res.in |
| Key Words |
Chronic liver diseases; Cell transplantation; Cell tracking and imaging; DiD; Hepatic progenitors |
| Core Tip |
Non-invasive tracking of transplanted cells is crucial to understand the homing, distribution, and differentiation into the desired cell types contributing to organ regeneration. Lipophilic fluorescent dye DiD-labeled fetal hepatic progenitor cells (fHPCs) were transplanted into chronic liver disease mice livers. DiD labeling of cells enabled long-term and non-invasive tracking of transplanted cells in vivo up to 80 d. Immunostaining and colocalization using liver-specific markers with DiD confirmed the persistence of transplanted cells in mice liver post-transplantation. Transplanted fHPCs supported liver function recovery while identifying the Alu gene sequence revealed survival and engraftment of human cells within the mice liver. |
| Publish Date |
2022-10-27 03:05 |
| Citation |
Tripura C, Gunda S, Vishwakarma SK, Thatipalli AR, Jose J, Jerald MK, Khan AA, Pande G. Long-term and non-invasive in vivo tracking of DiD-labeled human hepatic progenitors in chronic liver disease models. World J Hepatol 2022; 14(10): 1884-1898 |
| URL |
https://www.wjgnet.com/1948-5182/full/v14/i10/1884.htm |
| DOI |
https://dx.doi.org/10.4254/wjh.v14.i10.1884 |
