Publication Name	World Journal of Stem Cells
Manuscript ID	58632
Country	China

Received	2020-08-11 11:53
Peer-Review Started	2020-08-11 11:54
To Make the First Decision
Return for Revision	2020-10-23 21:07
Revised	2020-11-02 07:22
Second Decision	2020-11-17 12:17
Accepted by Journal Editor-in-Chief
Accepted by Company Editor-in-Chief	2020-11-17 22:50
Articles in Press	2020-11-17 22:50
Publication Fee Transferred
Edit the Manuscript by Language Editor	2020-11-30 23:55
Typeset the Manuscript	2021-01-06 02:59
Publish the Manuscript Online	2021-01-21 03:12

ISSN	1948-0210 (online)
Open Access	This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/Licenses/by-nc/4.0/
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Category	Cell Biology
Manuscript Type	Basic Study
Article Title	Proliferation and tenogenic differentiation of bone marrow mesenchymal stem cells in a porous collagen sponge scaffold
Manuscript Source	Unsolicited Manuscript
All Author List	Bing-Yu Zhang, Pu Xu, Qing Luo and Guan-Bin Song
ORCID	Author(s) ORCID Number Bing-Yu Zhang http://orcid.org/0000-0001-9859-4430 Pu Xu http://orcid.org/0000-0002-3785-7812 Qing Luo http://orcid.org/0000-0001-9329-0227 Guan-Bin Song http://orcid.org/0000-0001-5217-0296
Funding Agency and Grant Number	Funding Agency Grant Number Natural National Science Foundation of China No. 31700810 and 11772073 Science and Technology Research Program of Chongqing Municipal Education Commission No. KJQN201800601 Natural Science Foundation of Chongqing, China No. cstc2020jcyj-msxmX0760 Visiting Scholar Foundation of Key Laboratory of Biorheological Science and Technology (Chongqing University), Ministry of Education No. CQKLBST-2018-007
Corresponding Author	Guan-Bin Song, PhD, Professor, Department of College of Bioengineering, Chongqing University, No. 174 Shazhengjie, Shapingba, Chongqing 400030, China. song@cqu.edu.cn
Key Words	Bone marrow mesenchymal stem cells; Collagen sponge; Transforming growth factor β1; Tenogenic differentiation; Proliferation; Collagen deposition
Core Tip	Growth factor supplementation of stem cells facilitates cell differentiation and/or cell growth, but there is an incomplete understanding of which growth factors are optimal in 3D culture. We compared different growth factors for the proliferation and tenogenic differentiation of bone marrow mesenchymal stem cells (BMSCs) in a monolayer culture (2D) and in a collagen sponge-based 3D culture. We found that transforming growth factor β1 (TGF-β1) showed great promise in the tenogenic differentiation of BMSCs compared to growth differentiation factor 7 and insulin-like growth factor 1 in both 2D and 3D cultures, and the 3D culture enhanced the tenogenic differentiation of BMSCs well beyond the level of induction in the 2D culture after TGF-β1 treatment.
Publish Date	2021-01-21 03:12
Citation	Zhang BY, Xu P, Luo Q, Song GB. Proliferation and tenogenic differentiation of bone marrow mesenchymal stem cells in a porous collagen sponge scaffold. World J Stem Cells 2021; 13(1): 115-127
URL	https://www.wjgnet.com/1948-0210/full/v13/i1/115.htm
DOI	https://dx.doi.org/10.4252/wjsc.v13.i1.115

Full Article (PDF)	WJSC-13-115.pdf
Full Article (Word)	WJSC-13-115.docx
The ARRIVE Guidelines	58632-The-ARRIVE-Guidelines-revision_1626.pdf
Manuscript File	58632_Auto_Edited_WangTQ.docx
Answering Reviewers	58632-Answering reviewers.pdf
Audio Core Tip	58632-Audio core tip.mp3
Biostatistics Review Certificate	58632-Biostatistics statement.pdf
Conflict-of-Interest Disclosure Form	58632-Conflict-of-interest statement.pdf
Copyright License Agreement	58632-Copyright license agreement.pdf
Approved Grant Application Form(s) or Funding Agency Copy of any Approval Document(s)	58632-Grant application form(s).pdf
Institutional Animal Care and Use Committee Approval Form or Document	58632-Institutional animal care and use committee statement.pdf
Institutional Review Board Approval Form or Document	58632-Institutional review board statement.pdf
Non-Native Speakers of English Editing Certificate	58632-Language certificate.pdf
Peer-review Report	58632-Peer-review(s).pdf
Scientific Misconduct Check	58632-Bing-Huang p-1.png
Scientific Misconduct Check	58632-Bing-Fan JR-2.png
Scientific Misconduct Check	58632-Scientific misconduct check.pdf
Scientific Editor Work List	58632-Scientific editor work list.pdf